Multiplex preparation of barcoded gene specific DNA fragments

US11274334B2 · kind B2 · utility

Assignee

• Cellecta, Inc. · US

Inventors

• Alex Chenchik · Redwood City, US
• Costa Frangou · Sunnyvale, US
• Mikhail Makhanov · San Francisco, US
• Russell Paul Darst, IV · San Bruno, US
• Donato Tedesco · Mountain View, US
• Lester Kobzik · Mountain View, US

Key dates

• Filing date: Aug 16, 2019
• Grant date: Mar 15, 2022
• Expiry date: Nov 27, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods of preparing a plurality of sample-barcoded anchor-domain-flanked gene specific deoxyribonucleic acid (DNA) fragments from a template nucleic acid, e.g., ribonucleic acid (RNA), sample are provided. Aspects of the methods include employing a set of gene specific primer pairs, wherein each pair of gene specific primers is made up of a forward primer and a reverse primer, at least one of which includes a sample barcode domain. The methods find use in a variety of different applications, including high-throughput sequencing, e.g., expression profiling, applications, including of small biological samples, e.g., single-cells.