Patent · US Active

Enhanced ligation in sequencing library preparation

US11274344B2 · kind B2 · utility

0Cited by

15References

15Claims

0Family size

Assignee

GRAIL, INC. · US

Inventors

Craig Betts · Menlo Park, US
Byoungsok Jung · Palo Alto, US

Key dates

Filing date	Mar 30, 2018
Grant date	Mar 15, 2022
Priority date	—
Expiry date	Mar 30, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods for preparing a sequencing library from a DNA-containing test sample are provided. In some embodiments, the methods involve rescuing a partially ligated DNA fragment to enhance library preparation conversion efficiencies. In some embodiments, the methods involve improving recovery of duplex sequence information from double-stranded DNA.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.