Cytological staining compositions and uses thereof
US11274997B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 4, 2014 |
| Grant date | Mar 15, 2022 |
| Priority date | — |
| Expiry date | Mar 10, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/302
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present subject matter is directed to a composition for staining cytological material comprising a cationic dye component, a hygroscopic polyol and optionally a water-soluble solvent, water or water-miscible solvent, methods of use of the compositions. Advantageously, the present compositions do not require the use of a cover slide as is required in known staining fixatives. The compositions are able to retain cell morphology for a period of time such that a cover slide is not required. Further, the compositions do not contain hazardous levels of organic components. Preferably, the compositions consist essentially of Azure C, glycerol and optionally water. In another aspect, the present subject matter is directed to a method of characterizing a cell sample comprising contacting the cell sample with a composition comprising a dye component and a hygroscopic polyol and subjecting the sample to analysis to determine the presence or absence of abnormal cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.