Synthetic apolipoproteins, and related compositions methods and systems for nanolipoprotein particles formation

US11279749B2 · kind B2 · utility

Assignee

• Lawrence Livermore National Security, LLC · US

Inventors

• Paul D. Hoeprich, Jr. · Pleasanton, US
• Julio A. Camarero · Livermore, US

Key dates

• Filing date: Sep 9, 2016
• Grant date: Mar 22, 2022
• Expiry date: Sep 9, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G16C10/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Synthetic apolipoproteins based on native/naturally occurring homolog proteins can be prepared using solid-phase peptide synthesis approaches combined with native chemical ligation methods to create analogs of full length apolipoproteins. The chemical synthesis is expected to allow introduction of non-natural amino acids, e.g., α,α′-dialkyl amino acids, with a periodicity that encourages both helix formation and amphipathicity. Such apolipoprotein analogs are expected to encourage, in some embodiments, facile and more complete NLP formation, enabling consideration of full spectrum of nanoparticle-based biotechnology applications ranging from therapeutic sequestration and delivery to energy/biofuel production to biopolymer production.