Patent · US Active

Compositions and methods for constructing strand specific CDNA libraries

US11279927B2 · kind B2 · utility

0Cited by

1References

16Claims

0Family size

Assignee

The Regents of the University of California · US

Inventors

Brad Townsley · Oakland, US
Michael F. Covington · Oakland, US
Neelima Sinha · Pune, IN

Key dates

Filing date	Apr 29, 2016
Grant date	Mar 22, 2022
Priority date	—
Expiry date	Apr 29, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC40B40/08
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided herein are compositions, kits and methods for the production of strand-specific cDNA libraries. The compositions, kits and methods utilize properties of double stranded polynucleotides, such as RNA-cDNA duplexes to capture and incorporate a novel sequencing adapter. The methods are useful transcriptome profiling by massive parallel sequence, such as full-length RNA sequencing (RNA-Seq) and 3′ tag digital gene expression (DGE).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.