Patent · US Active

System and method for identification and characterization of transglutaminase species

US11280791B2 · kind B2 · utility

0Cited by

0References

8Claims

0Family size

Assignee

Roche Sequencing Solutions, Inc. · US

Inventors

Thomas Albert · Danville, US
Frank Bergmann · Iffeldorf, DE
Victor Lyamichev · Madison, US
Mohamed Yosry Hassan Mohamed · Munich, DE
Tobias Oelschlaegel · Munich, DE
Jigar Patel · London, GB
Michael Schraeml · Penzberg, DE
Wojtek Steffen · Rosenheim, DE
Thomas Streidl · Seeshaupt, DE

Key dates

Filing date	Aug 12, 2020
Grant date	Mar 22, 2022
Priority date	—
Expiry date	Aug 12, 2040

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/91085
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.