Reprogramming of aged adult stem cells
US11286463B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 26, 2015 |
| Grant date | Mar 29, 2022 |
| Priority date | — |
| Expiry date | Oct 26, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/84
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method comprising (a) obtaining young adult stem cells (YASC) from a first subject; (b) lysing the YASC to generate a lysate comprising an intracellular matrix (ICM) and other cellular components; (c) filtering the lysate through a filter having a membrane size of equal to or less than 0.4 micron to obtain a filtrate; and (d) applying the filtrate to a culture of aged adult stem cells (AASC) for a time period ranging from about 24 hours to about 30 days to generate a reprogrammed AASC (R-AASC) wherein the AASC were obtained from a second subject and wherein the R-AASC when infused into a breast cancer stem model results in a reduced number of breast cancer cells in bone marrow in comparison to a breast cancer stem model having AASC that have not been reprogrammed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.