Preparation of libraries of protein variants expressed in eukaryotic cells and use for selecting binding molecules
US11286477B2 · kind B2 · utility
2Cited by
10References
15Claims
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Key dates
| Filing date | May 1, 2015 |
| Grant date | Mar 29, 2022 |
| Priority date | — |
| Expiry date | Jun 24, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/80
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.