Method to produce protein in Penicillium amagasakiense's sleeping spores by transformation of ssRNA
US11286504B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 8, 2019 |
| Grant date | Mar 29, 2022 |
| Priority date | — |
| Expiry date | Jul 31, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses A METHOD TO PRODUCE PROTEIN IN PENICILLIUM AMAGASAKIENSE'S SLEEPING SPORES BY TRANSFORMATION OF SSRNA. The method includes three steps of culture of Penicillium amagasakiense and collection of spores, pretreatment of Penicillium anagasakiense spores, and electroporation of Penicillium anagasakiense spores by using HDEN method. In the present invention, non-germinated spores are used as a starting material for introduction of exogenous molecules. The exogenous protein coding single stranded RNA is introduced into the resting spores of Penicillium amagasakiense by employing the HDEN electrotransformation technique to express protein. The method of this invention is simple and fast, the effect is excellent, and the transformation rate reaches more than 90%.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.