Patent · US Active

Method to produce protein in Aspergillus niger's resting spores using SSRNA

US11286505B2 · kind B2 · utility

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6Claims
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Inventor

Key dates

Filing dateMar 8, 2019
Grant dateMar 29, 2022
Priority date—
Expiry dateMar 8, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12R2001/685
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method to produce protein in Aspergillus niger's sleeping spores using single-stranded RNA is provided. The method includes three steps: culture of Aspergillus niger and collection of spores, pretreatment of Aspergillus niger spores, and electroporation of Aspergillus niger spores using HDEN method. Non-germinated spores are used as a starting material for introduction of exogenous molecules. The exogenous protein coding single-stranded RNA is introduced into the resting spores of Aspergillus niger by employing the HDEN electrotransformation technique to express protein. This method is simple and fast, the effect is excellent, and the transformation rate reaches more than 90%.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.