Methods and compositions for enrichment of amplification products

US11286519B2 · kind B2 · utility

Assignee

• ACCURAGEN HOLDINGS LIMITED · KY

Inventors

• Li Weng · Fremont, US
• Shengrong Lin · Hayward, US
• Lin Tang · San Francisco, US

Key dates

• Filing date: Jun 7, 2019
• Grant date: Mar 29, 2022
• Expiry date: Jun 7, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.