Multivalent binding composition for nucleic acid analysis

US11287422B2 · kind B2 · utility

Assignee

• Element Biosciences, Inc. · US

Inventors

• Michael Previte · San Diego, US
• Molly He · San Diego, US
• Junhua ZHAO · San Diego, US
• Hui Zhen MAH · San Diego, US
• Chunhong Zhou · Pasadena, US
• Sinan ARSLAN · San Diego, US
• Matthew KELLINGER · San Diego, US
• Lorenzo Berti · San Diego, US
• Steve Xiangling CHEN · San Diego, US

Key dates

• Filing date: Jun 24, 2021
• Grant date: Mar 29, 2022
• Expiry date: Jun 24, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.