Application of viable cell monitor to cryopreservation of plant cells
US11291200B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 2017 |
| Grant date | Apr 5, 2022 |
| Priority date | — |
| Expiry date | Sep 23, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/22
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates to the application of a viable cell monitor to the cryopreservation of plant cells. The viable cell monitor is, for the first time, used in a method for rapidly evaluating the effect of cell freezing-preservation. The effect of a cryoprotectant on cell preservation is evaluated through the change in the capacitance of viable cells before and after cell freezing and resuscitation. Viable cell electrodes can provide a quick and accurate characterization of viable cell concentrations. Combining the viable cell capacitance with the detection result of 2,3,4-triphenyl tetrazolium chloride (TTC), a rapid and quantitative evaluation of the effect of a freezing method on cell preservation can be achieved, thus overcoming the low effectiveness and non-quantification of previous cell preservation methods. Compared with traditional methods, the method of the present disclosure can improve the comprehensive score of cell cryopreservation by 5 times or more.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.