Gene sequencing chip and gene sequencing method
US11291970B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 2, 2018 |
| Grant date | Apr 5, 2022 |
| Priority date | — |
| Expiry date | Apr 25, 2040 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0427
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A gene sequencing chip is provided, which includes: an upper substrate including a plurality of liquid inlets for inletting liquid drops; a lower substrate opposite to the upper substrate and spaced therefrom by a gap, the gap being provided for allowing the liquid drops to move therein, the lower substrate including a liquid drop operation region, the liquid drop operation region including a manipulation electrode array. The manipulation electrode array includes multiple first sub-arrays for preparing a gene library, multiple second sub-arrays for sequencing the gene library which is prepared, each first sub-array being adjacent to one of the multiple second sub-arrays. Based on the gene sequencing chip provided in this disclosure, operations to tiny liquid drops such as movement, fusion and splitting can be accurately manipulated by using digital microfluidic techniques, and all steps of the gene sequencing from library preparation to gene sequencing can be completed on one chip.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.