Methods for amplification and sequencing using thermostable TthPrimPol
US11299718B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Mar 14, 2014 |
| Grant date | Apr 12, 2022 |
| Priority date | — |
| Expiry date | Aug 9, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to methods for replicating, amplifying, and sequencing of nucleic acids using the thermostable, bifunctional replicase “TthPrimPol” from Thermus thermophilus HB27. The TthPrimPol enzyme is extremely tolerant to alterations of the nucleotides of the template nucleic acid. Therefore, in one aspect the invention discloses methods for replicating, amplifying and sequencing of damaged polynucleotide templates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.