Acid phosphatase mutant, use thereof and method for preparing nicotinamide riboside by same
US11299721B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 17, 2018 |
| Grant date | Apr 12, 2022 |
| Priority date | — |
| Expiry date | Dec 11, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/03002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An acid phosphatase mutant, and method for preparing nicotinamide riboside by same. The mutant is a protein of the following (a), (b) or (c): (a) a protein, having an amino acid sequence shown in SEQ ID NO: 3; (b) a protein, derived from (a), having catalytic activity higher than an acid phosphatase parent having an amino acid sequence shown in SEQ ID NO: 2, obtained by substituting, deleting or adding several amino acids in the amino acid sequence shown in SEQ ID NO: 3, using nicotinamide mononucleotide as a substrate; (c) a protein, having catalytic activity higher than the acid phosphatase shown in SEQ ID NO: 2, having 90% or more homology with the amino acid sequence of the protein defined by (a) or (b), and using nicotinamide mononucleotide as a substrate. It is used to prepare nicotinamide riboside and the conversion rate can be 99%.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.