Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US11299774B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 20, 2021 |
| Grant date | Apr 12, 2022 |
| Priority date | — |
| Expiry date | Sep 20, 2041 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/163
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.