Methods and systems for producing nanolipoprotein particles

US11300572B2 · kind B2 · utility

Assignee

• Lawrence Livermore National Security, LLC · US

Inventors

• Matthew A. Coleman · Dallas, US
• Paul D. Hoeprich, Jr. · Pleasanton, US
• Brent W. Segelke · San Ramon, US

Key dates

• Filing date: Oct 12, 2018
• Grant date: Apr 12, 2022
• Expiry date: May 30, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K17/02
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein are methods and systems for the production of a nanolipoprotein particle (NLP) that includes a scaffold protein a membrane forming lipid and optionally a target protein. At least one of the scaffold protein and target protein can be provided through an IVT system. The membrane forming lipid, scaffold protein and optionally the target protein can be assembled for a time and under conditions that allow obtaining high yield NLPs, NPLs with an increased solubility, an NLP of a controlled size, and/or an NLP having a size predetermined to include a pre-selected target protein.