Composite multi-epitope expression cassette, a recombinant virus composed thereof and application thereof

US11305007B2 · kind B2 · utility

Assignee

• SHANGHAI VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES (NATIONAL CENTER FOR ANIMAI HEALTH SHANGHAI) · CN

Inventors

• Chan Ding · Shanghai, CN
• Huabin Shao · Shanghai, CN
• Lei Tan · Beijing, CN
• Guoyuan Wen · 红钢城街道, CN
• Shengqing Yu · Shanghai, CN
• Xusheng Qiu · Shanghai, CN
• Ying-Jyh Liao · Taipei, TW
• Chunchun Meng · Shanghai, CN
• Yingjie Sun · Irvine, US
• Cuiping Song · Shanghai, CN

Key dates

• Filing date: Jan 21, 2019
• Grant date: Apr 19, 2022
• Expiry date: Mar 29, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/20034
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present application relates a composite multi-epitope expression cassette, a recombinant virus composed thereof and application thereof, and in particular to a chimeric recombinant Newcastle disease virus inserted with an IBV epitope cassette and a vaccine prepared by using the virus. The expression cassette comprises: (a) T cell epitopes derived from S1 proteins of avian infectious bronchitis virus Holte strain and avian infectious bronchitis virus QX-like strain; and (b) B cell epitopes derived from S1 protein of avian infectious bronchitis virus Australian T strain. In the present application, the multi-epitope chimeric ST/B gene of avian infectious bronchitis virus is inserted into the backbone of LaSota strain, so that the LaSota strain can express S1-T/B protein. Thus, the purpose of preventing both ND and IB diseases is achieved. In addition, the T cell epitopes and B cell epitopes act synergistically to produce an earlier and more comprehensive immune response against virus.