Method for automated detection of antibodies in a liquid biological sample using an antigen chip, and antigen chip therefor

US11305289B2 · kind B2 · utility

Assignee

• EUROIMMUN Medizinische Labordiagnostika AG · DE

Inventors

• Katja Morgenroth · Herrnburg, DE
• Vanessa Viertel · Stade, DE
• Ulf Steller · Kürten, DE
• Stefan Gerlach · Groß, DE
• Christian Marzahl · Erlangen, DE
• Jörn Voigt · Lübeck, DE
• Winfried Stöcker · Erkrath, DE

Key dates

• Filing date: Jun 17, 2019
• Grant date: Apr 19, 2022
• Expiry date: Jun 17, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6439
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method is useful for automated detection of antibodies in a liquid biological sample with an antigen chip having antigen spots which are applied thereon and which have an identical, common dye. The antigen spots form respective antigen spot sets which form corresponding respective, regular antigen spot patterns. Furthermore, reference spots comprising the identical dye are applied, and form a reference spot set which forms a regular reference pattern. The reference pattern differs with respect to its regularity from the antigen spot patterns. Through a first image information item which represents a color of the reference spots and of the antigen spots due to the identical, first dye and through a second image information item which represents a potential color of the antigen spots due to a second dye after an incubation, binding of antibodies of the biological sample to respective antigen types is then determined by image processing.