Genome engineering

US11306328B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• George M. Church · Brookline, US
• Luhan Yang · Somerville, US
• Marc Guell · Boston, US
• Joyce Lichi Yang · Cambridge, US

Key dates

• Filing date: Jun 30, 2014
• Grant date: Apr 19, 2022
• Expiry date: Jun 30, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods are provided for altering target DNA in a cell genetically modified to express a Cas 9 enzyme that forms a co-localization complex with a guide RNA complementary to the target DNA and that cleaves the target DNA in a site specific manner. Methods include introducing into the cell a first foreign nucleic acid encoding a donor nucleic acid sequence, introducing into the cell from media surrounding the cell the guide RNA complementary to the target DNA and which guides the Cas 9 enzyme to the target DNA, wherein the RNA and the enzyme are members of a co-localization complex for the target DNA, wherein the donor nucleic acid sequence is expressed, wherein the guide RNA and the Cas 9 enzyme co-localize to the target DNA, the Cas 9 enzyme cleaves the target DNA and the donor nucleic acid is inserted into the target DNA to produce altered DNA in the cell.