Methods of culturing a mammalian cell
US11306341B2 · kind B2 · utility
2Cited by
4References
13Claims
0Family size
Assignee
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Key dates
| Filing date | Dec 21, 2015 |
| Grant date | Apr 19, 2022 |
| Priority date | — |
| Expiry date | Nov 29, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.