Patent · US Active

In vitro and cell based assays for measuring the activity of botulinum neurotoxins

US11306347B2 · kind B2 · utility

1Cited by
10References
3Claims
0Family size

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Inventors

Key dates

Filing dateOct 19, 2017
Grant dateApr 19, 2022
Priority date—
Expiry dateMar 30, 2038

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/542
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Disclosed herein are means for the detection and characterization of neurotoxins such as botulinum neurotoxin (BoNT) or tetanus neurotoxin. The present disclosure provides methods for determining potency and activity of neurotoxins in vitro and in vivo. Also disclosed are polypeptides comprising N- and C-terminal fragments of a reporter protein that are split by a linker comprising a neurotoxin cleavage site. Cleavage of the linker by a neurotoxin decreases reporter protein activity, thereby indicating activity of the neurotoxin. Compositions and kits comprising the disclosed polypeptides, nucleic acids comprising nucleotide sequences encoding such polypeptides, and cells expressing such polypeptides are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.