Biotinylated and oxidized LDL receptor and advanced glycation end product receptor produced using genetically engineered silkworm
US11311002B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 1, 2015 |
| Grant date | Apr 26, 2022 |
| Priority date | — |
| Expiry date | Nov 14, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/323
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.