Method and enzyme for preparation of enzyme-modified stevia sugar and use of enzyme-modified stevia sugar
US11312948B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 25, 2018 |
| Grant date | Apr 26, 2022 |
| Priority date | — |
| Expiry date | Apr 24, 2039 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA23V2300/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for preparation of an enzyme-modified stevia sugar. The method includes the steps of adding a β-fructosidase to a solution in which a stevia sugar raw material and sucrose are dissolved to obtain a reaction solution, adjusting the pH of the reaction solution to be 5.0-8.0, maintaining a reaction temperature at 20-45° C., and after a reaction with stirring, collecting the enzyme-modified stevia sugar. The stevia sugar raw material includes one or more of stevioside and rebaudioside A, and the β-fructosidase is derived from Microbacterium saccharophilum or Aspergillus japonicus. The preparation method takes a short time, is efficient and convenient to operate, low in cost, high in conversion rate, green and environmentally friendly, and can be widely applied to industrial scale production. The present invention further provides an enzyme for preparation of the enzyme-modified stevia sugar and application thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.