Method for detecting SNP site on SMA gene
US11312995B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 5, 2018 |
| Grant date | Apr 26, 2022 |
| Priority date | — |
| Expiry date | Jun 18, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting a SNP site on a SMA gene is disclosed, and includes steps of: (S10) performing a PCR for amplifying a nucleic a nucleic acid fragment containing a SNP site; (S20) performing a dephosphorylation reaction on the nucleic acid fragment; (S30) performing an extension reaction on the nucleic acid fragment, wherein the SNP site is identified by using an extension primer, a 3′-end of the extension primer is extended by a single nucleotide which is complementary to a base of the SNP site, and thus an extended extension primer is obtained; (S40) performing a purification reaction; and (S50) measuring a molecular weight of the extended extension primer, and determining a type of a base of the single nucleotide based upon the molecular weight, thereby determining whether deletion occurs to the SNP site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.