Live-cell computed tomography
US11315292B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Mar 2, 2018 |
| Grant date | Apr 26, 2022 |
| Priority date | — |
| Expiry date | Oct 5, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
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