Soluble and immunoreactive flaviviral NS1 polypeptides comprising the wing domain

US11319351B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Elke Faatz · Huglfing, DE
• Alexander Riedel · Munich, DE
• Christian Scholz · Penzberg, DE
• Peter Muench · Penzberg, DE
• Gloria Tabares · Penzberg, DE
• Mario Gloeck · Geretsried, DE
• Silke Luebcke · Mainz, DE
• Juliane Benz · Weilheim, DE

Key dates

• Filing date: Oct 25, 2019
• Grant date: May 3, 2022
• Expiry date: Feb 13, 2040

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The disclosure relates to a polypeptide suitable for detecting antibodies against a flavivirus in an isolated biological sample having a flavivirus NS1 wing domain specific amino acid sequence, wherein no amino acid sequences from the NS1 ß-ladder domain of said flavivirus are present in the polypeptide. In an embodiment, the flavivirus is selected from Zika virus (ZIKV), West-Nile virus (WNV), Dengue virus types 1-4 (DENV1-4), tick-borne encephalitis virus (TBEV), yellow fever virus (YFV) and Japanese encephalitis virus (JEV). Also disclosed is a method for producing said flaviviral NS1 wing domain specific polypeptides, a method for detecting antibodies specific for a first flavivirus species, the use of said flaviviral NS1 wing domain specific polypeptides for detecting antibodies as well as a reagent kit for detecting said flavivirus antibodies that has a flavivirus NS1 wing domain polypeptide.