Methods for increasing observed editing in bacteria
US11319542B2 · kind B2 · utility
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24References
20Claims
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Key dates
| Filing date | Nov 28, 2021 |
| Grant date | May 3, 2022 |
| Priority date | — |
| Expiry date | Nov 28, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.