L-isoleucine-producing corynebacterium glutamicum fermentation medium and culture method

US11319563B2 · kind B2 · utility

Assignee

• WUHAN GRAND HOYO CO., LTD. · CN

Inventors

• Xuechen Mei · 红钢城街道, CN
• Jiong Wang · Union City, US
• Kun Wan · 红钢城街道, CN
• Mengjun Song · 红钢城街道, CN
• Panpan XING · 红钢城街道, CN
• Haixia SU · 红钢城街道, CN
• Jing Li · Lexington, US
• Aifu Liu · 红钢城街道, CN

Key dates

• Filing date: Dec 1, 2017
• Grant date: May 3, 2022
• Expiry date: Feb 5, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2500/76
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is a L-isoleucine-producing Corynebacterium glutamicum fermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. Also disclosed is a method for cultivating the L-isoleucine-producing Corynebacterium glutamicum, comprising: inoculating the L-isoleucine-producing Corynebacterium glutamicum onto the fermentation medium, wherein the volume of the bacteria liquid accounts for 5-20% of the volume of the fermentation medium, adjusting the pH to 6.5-7 with aqueous ammonia, controlling the dissolved oxygen to 30-50%, and fermenting for 25-30 h; then decreasing the dissolved oxygen to 15-25%, and feeding a 50-80% glucose solution into the fermentation broth to control the residual sugar at 3-4%, continuing the fermentation until 60-70 hours, then terminating the fermentation, and controlling the temperature of the overall fermentation process at 29-33° C.