Cucumber male sterility gene, molecular marker, screening method and application thereof

US11319600B2 · kind B2 · utility

Assignee

• Tianjin Kernel Agricultural Science and Technology Corporation Ltd. Cucumber Research Institute · CN

Inventors

• Yike Han · Tianjin, CN
• Shengli Du · Tianjin, CN
• Fengyue Zhao · Tianjin, CN
• Aimin Wei · Tianjin, CN
• Nan Liu · Nanjing, CN
• Zhengwu Chen · Tianjin, CN
• Xinmeng Fu · Tianjin, CN

Key dates

• Filing date: Jan 12, 2018
• Grant date: May 3, 2022
• Expiry date: Jun 29, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relate to SNP and InDel markers associated with a cucumber male sterility gene and a screening method thereof. The cucumber male sterility gene is acquired by screening according to the markers. Specifically, the screening method includes following steps: (1) configuring groups; (2) building a library and sequencing; (3) adopting bulked segregation analysis (BSA); and (4) performing competitive allele specificity PCR (KASP), finely mapping a sterility gene, and acquiring SNP or InDel locus in close linkage with sterility character. The acquiring of the male sterility gene provides an effective tool for quick identification of cucumber male sterility plants and efficient production of cucumber hybrid species.