Patent · US Active

Combination of reversible and irreversible cell labeling for analizing receptor-ligand koff rate

US11320431B2 · kind B2 · utility

0Cited by

8References

19Claims

0Family size

Assignees

TECHNISCHE UNIVERSITÄT MÜNCHEN · DE
Juno Therapeutics, Inc. · US

Inventors

Magdalena NAUERTH · Munich, DE
Dirk Busch · Munich, DE
Lothar GERMEROTH · Oettingen, DE

Key dates

Filing date	Jun 27, 2017
Grant date	May 3, 2022
Priority date	—
Expiry date	Mar 5, 2038

Classification

Technology area (CPC G)Physics
CPC primaryG01N2458/00
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention relates to a method of determining the dissociation rate constant (koff) of a receptor molecule R on a target cell using a combination of reversible and irreversible cell labeling. The invention further relates to a cell comprising such a receptor molecule R, wherein the cell has bound to it such a combination of cell labeling. The invention further relates to a kit and an apparatus useful in performing the methods of the invention. The invention further relates to a method of isolation a high-avidity T cell.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.