Anti third party central memory T cells, methods of producing same and use of same in transplantation and disease treatment

US11324777B2 · kind B2 · utility

Assignee

• YEDA RESEARCH AND DEVELOPMENT CO. LTD. · IL

Inventors

• Yair Reisner · Jaffa, IL
• Yaki Eidelstein · Rehovot, IL
• Eran Ophir · Rehovot, IL
• Assaf Lask · Rehovot, IL
• Ran Afik · Rehovot, IL
• Noga Or-Geva · Rehovot, IL
• Esther Bachar-Lustig · Rehovot, IL

Key dates

• Filing date: Nov 29, 2017
• Grant date: May 10, 2022
• Expiry date: Jan 17, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2502/1121
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method of generating an isolated population of cells comprising anti-third party cells having a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance-inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) contacting peripheral blood mononuclear cells (PBMC) with a third party antigen or antigens in the presence of IL-21 so as to allow enrichment of antigen reactive cells; and (b) culturing the cells resulting from step (a) in the presence of IL-21, IL-15 and IL-7 in an antigen free environment so as to allow proliferation of cells comprising the central memory T-lymphocyte (Tcm) phenotype.