Pretreatment method for rapid detection of HCV core antigen

US11327077B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Barbara Upmeier · Iffeldorf, DE
• Toralf Zarnt · Penzberg, DE
• Manfred Ginter · Wielenbach, DE
• Ralf Bollhagen · Penzberg, DE

Key dates

• Filing date: Nov 29, 2018
• Grant date: May 10, 2022
• Expiry date: Nov 29, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/84
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure relates to a method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject with the steps of (a) contacting the sample with a surfactant comprising a cationic detergent; (b) contacting the sample with a binding compound; and (c) detecting a core polypeptide of the HCV in the sample; wherein step a) is immediately followed by step b). The present disclosure further relates to a method for pre-processing a sample from a subject for detection of an HCV core polypeptide, involving (a) contacting the sample with a surfactant comprising a cationic detergent and, optionally, with an agent inducing a pH shift, immediately followed by (b) contacting the sample with a binding compound. Moreover, the present disclosure further relates to uses, devices, and analytical systems related to aforesaid methods.