Methods and compositions for rapid plant transformation

US11330776B2 · kind B2 · utility

Assignee

• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• AJITH ANAND · West Des Moines, US
• MAREN L ARLING · Des Moines, US
• ALEXANDRE DA SILVA CONCEICAO · Wilmington, US
• William J. Gordon-Kamm · Urbandale, US
• Craig Hastings · Perry, US
• George J. Hoerster · Des Moines, US
• TODD J JONES · Kent, US
• Theodore M. Klein · Wilmington, US
• CARLOS M LA ROTA · Johnston, US
• Keith Lowe · Johnston, US
• Shiv B. TIWARI · San Jose, US
• Ning Wang · 红钢城街道, CN
• Xinli E. Wu · Johnston, US

Key dates

• Filing date: Aug 26, 2016
• Grant date: May 17, 2022
• Expiry date: Aug 26, 2036

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.