Nitrile hydratase mutant, genetically engineered bacterium containing mutant and applications thereof
US11332731B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 2021 |
| Grant date | May 17, 2022 |
| Priority date | — |
| Expiry date | Mar 22, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y402/01084
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure discloses a nitrile hydratase mutant, a genetically engineered bacterium containing the mutant and applications thereof, and belongs to the technical field of enzyme engineering. In the disclosure, glycine at position 47 of a nitrile hydratase mutant αL6T/A19V/F126Y-βM46K/E108R/S212Y (disclosed in the patent of disclosure CN102216455A) is mutated to asparagine. The obtained new mutant enzyme has better temperature tolerance and tolerance to a product, and is conducive to future industrial production. The recombinant strain containing the nitrile hydratase mutant is fermented at high density, and 3-cyanopyridine is used as a substrate to carry out a whole-cell catalytic reaction to prepare nicotinamide. Compared with a chemical production method, the method has a safe and clean production process and no environmental pollution. Compared with an enzymatic method, the substrate price is cheap and the catalytic efficiency is high. The yield of the final product nicotinamide is over 95%, the concentration reaches 680 g/L, and the separation and purification steps of the product are simplified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.