Nucleic acid melting analysis with saturation dyes
US11332779B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Apr 9, 2020 |
| Grant date | May 17, 2022 |
| Priority date | — |
| Expiry date | Apr 18, 2040 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.