Fitting melting curve data to determine copy number variation
US11332781B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 3, 2017 |
| Grant date | May 17, 2022 |
| Priority date | — |
| Expiry date | Nov 10, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B45/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method and system for determining Copy Number Variations (CNVs) in a genomic test sample including target amplicons and a reference amplicons. Specifically, nucleic acid melting curves are generated for the test sample. A mathematical model is fitted to each of the nucleic acid melting curves to separate target and reference melting reactions within the measured nucleic acid melting curve. The fitting parameters of the mathematical model are calculated. A CNV of the test sample is determined based on the fitting parameters of the mathematical model corresponding to the target and reference melting reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.