Nanopore sensor for enzyme-mediated protein translocation
US11339365B2 · kind B2 · utility
2Cited by
5References
17Claims
0Family size
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Key dates
| Filing date | Feb 15, 2013 |
| Grant date | May 24, 2022 |
| Priority date | — |
| Expiry date | Feb 15, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/48721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be translocated. The exemplified unfoldase is the ClpX unfoldase from E. coli.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.