SO3 chromatography for use in a method for virus purification
US11339377B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Nov 8, 2018 |
| Grant date | May 24, 2022 |
| Priority date | — |
| Expiry date | Nov 8, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/16252
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.