Methods of analyzing nucleic acids

US11339422B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Scott Kuersten · Madison, US
• Agnes Radek · Madison, US
• Ramesh Vaidyanathan · Madison, US
• Haiying Li Grunenwald · Belleville, US

Key dates

• Filing date: Mar 26, 2020
• Grant date: May 24, 2022
• Expiry date: Jul 22, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).