Patent · US Active

High pH protein refolding methods

US11345722B2 · kind B2 · utility

0Cited by

20References

14Claims

0Family size

Assignee

Bristol-Myers Squibb Company · US

Inventors

Cristopher Hollander · Newton, US
Benjamin C. Blum · Boston, US

Key dates

Filing date	Jul 31, 2018
Grant date	May 31, 2022
Priority date	—
Expiry date	Oct 10, 2040

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/70
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided herein are methods for refolding denatured protein (e.g., from inclusion bodies) that do not require the use of a denaturing agent. Exemplary methods use a high pH for solubilizing denatured protein, followed by a decrease in pH for refolding the proteins.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.