Rapid diagnostic test for LAMP
US11345970B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 19, 2021 |
| Grant date | May 31, 2022 |
| Priority date | — |
| Expiry date | Aug 19, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/78
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.