Aureobasidium pullulans strains with high-yield heavy oil and construction method and application thereof

US11352633B2 · kind B2 · utility

Assignee

• Tianjin University of Science and Technology · CN

Inventors

• Yefu Chen · Tianjin, CN
• Siyao Huang · Tianjin, CN
• Peng Zheng · Tianjin, CN
• Yuanhua Wang · Tianjin, CN
• Jian Guo · Jiaocheng District, CN
• Mengjuan Zhang · Tianjin, CN
• Xuewu Guo · Tianjin, CN
• Dongguang Xiao · Tianjin, CN

Key dates

• Filing date: Oct 15, 2020
• Grant date: Jun 7, 2022
• Expiry date: Oct 15, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/645
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An Aureobasidium pullulans recombinant strain with high-yield heavy oil and a construction method and application thereof are provided. The Aureobasidium pullulans recombinant strain is obtained by knocking out a pullulan synthetase PUL gene while overexpressing an ACL gene. The obtained Aureobasidium pullulans recombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.