Methods of detecting analytes

US11352659B2 · kind B2 · utility

Assignee

• Spatial Transcriptomics AB · SE

Inventors

• Jonas Frisen · Stockholm, SE
• Patrik Stahl · Stockholm, SE
• Joakim Lundeberg · Lidingö, SE

Key dates

• Filing date: Sep 14, 2021
• Grant date: Jun 7, 2022
• Expiry date: Sep 14, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y600/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.