Sequence adapter manufacture and use
US11352662B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jan 22, 2018 |
| Grant date | Jun 7, 2022 |
| Priority date | — |
| Expiry date | Jan 22, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Technology provided herein relates in part to methods, processes, machines and apparatuses for determining sequences of nucleotides for nucleic acid templates in a nucleic acid sample. The technology provide herein also relates in part to methods, processes, machines and apparatuses for counting nucleic acid templates. Nucleic acid templates of a sample are tagged with nonrandom oligonucleotide adapters that include predetermined non-randomly generated sequences. The use of these nonrandom oligonucleotide adapters provides an efficient method to reduce sequencing errors, and increase the sensitivity of detection of low-frequency single nucleotide alterations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.