Method of screening tobacco germplasm for resistance to Alternaria alternata by ripening seedling leaves
US11357178B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 2021 |
| Grant date | Jun 14, 2022 |
| Priority date | — |
| Expiry date | Mar 14, 2041 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P60/21
- WIPO fieldOther special machines
- WIPO sectorMechanical engineering
Abstract
The disclosure provides a method of screening tobacco germplasm for resistance to Alternaria alternata by ripening seedling leaves. The method includes ripening seedling leaves, spray inoculation, disease induction, and evaluation of disease resistance. Dense planting, fertilizer control, and potassium increment were used to forcibly ripening seedling leaves. A hospitable environment was simulated to induce disease in the ripened leaves. These treatments reduce differences in leaf maturity and avoid environmental changes. The technique of the disclosure provides greater accuracy and repeatability than the current technique of screening brown spot resistance, and offers the advantages of simple operation, reduced cost, space requirement, and labor intensity, high selection efficiency, and an accurate screening of tobacco phenotypes with resistance to brown spot, etc., used for large-scale screening of tobacco varieties with resistance to brown spot.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.