Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
US11359221B2 · kind B2 · utility
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Key dates
| Filing date | Feb 11, 2020 |
| Grant date | Jun 14, 2022 |
| Priority date | — |
| Expiry date | Feb 11, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using endonuclease V activity and initiator with a 3′-penultimate deoxyinosine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.