Automated analysis tool for biological specimens

US11360025B2 · kind B2 · utility

Assignee

• MILTENYI BIOTEC B.V. & CO. KG · DE

Inventors

• Thomas Rockel · Mülheim an der Ruhr, DE
• Stefan Miltenyi · Wittlich, DE
• Sven Ignatius · Bensberg, DE
• Jurgen Krieg · Cologne, DE

Key dates

• Filing date: Nov 28, 2017
• Grant date: Jun 14, 2022
• Expiry date: Jul 31, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2201/061
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Systems and methods are described for analyzing a plurality of biological samples with a plurality of fluorescent reagents in an automated fashion. The system may include two optical systems, a fluorescence imaging system and an optical quenching system. These two systems may be placed laterally adjacent to one another, and generally beneath one of the wells of sample-containing microtiter plate. The biological sample contained therein may be stained with a series of fluorescent reagents, with the fluorescence quenched between stainings. By precise positioning of the sample with respect to the imaging system, the sample may be imaged with a plurality of serially applied reagents.