Methods for selecting phosphatase selective and non-selective phosphatase inhibitors
US11364211B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 8, 2016 |
| Grant date | Jun 21, 2022 |
| Priority date | — |
| Expiry date | Apr 8, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/916
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention discloses a method to discover selective inhibitors of phosphatases. Thus the invention provides a method for screening a test compound to determine whether the compound binds a holophosphatase selectively or non-selectively comprising: i) providing a first holophosphatase wherein said holophosphatase is captured/immobilised; ii) testing a test compound for its ability to bind to the first holophosphatase; iii) providing a second holophosphatase wherein said second holophosphatase is captured/immobilised; iv) testing the same test compound for its ability to bind to the second holophosphatase; v) comparing the binding of the test compound to said first holophosphatase with the binding to said second phosphatase wherein a compound that binds a holophosphatase selectively will bind to said first holophosphatase but not said second holophosphatase; or will bind to said second holophosphatase but not said first; or wherein a compound that binds a holophosphatase non-selectively will bind to both said first holophosphatase and said second holophosphatase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.